Genetic Engineering
Genetic Engineering: Overview
This Topic covers sub-topics such as Restriction Endonuclease, Genetic Engineering, Human Genome Project, Cloning Vectors, Genetic Mapping, DNA Ligase, Genetically Engineered Insulin and, Gene Therapy
Important Questions on Genetic Engineering
Which of the following enzyme plays role in only the formation of discontinuous strand of DNA?
The DNA ligase facilitates the joining of DNA strands together by catalysing the formation of a phosphodiester bond.
Describe the gene therapy? Write the uses of gene therapy.
Explain Restriction endonuclease.
Fill in the blank with the correct option from the bracket.
The application of genetically modified pathogens on enemies is called _____. (biowar, gene therapy, DNA fingerprinting)
The majority of genes in human DNA are junk genes.
Gene therapy is the method of treatment of contagious diseases.
Fill in the blank with the correct option from the bracket.
Only _____ percent difference in DNA among humans. (2, 20, 0.2)
The human genome project was started in 1995 and completed in 2000.
Name the method of treatment in which the genes that are responsible for diseases are removed and normal functional genes are inserted in their place, whether it is radiation therapy or gene therapy.
Cloning vectors are called vehicle DNA.
Restriction endonuclease is an enzyme commonly known as genetic glue.
Name the enzyme which is used to join DNA fragments, whether it is endonuclease or ligase.
The plasmid is extracted from bacteria that can be used to cut DNA at specific locations.
The extrachromosomal circular DNA of bacteria used as a vector in genetic engineering is called _____.
The genetically modified bacteria are used for the commercial production of insulin.
Fill in the blank with the correct word given in the bracket.
Two polypeptide chains in insulin is connected by _____ (hydrogen/disulphide) bonds.
Insulin is a branched polypeptide that consists of three different polypeptide chains.
Vectors that are constructed by combining a certain sequence of plasmid and 'cos' site of phage lambda called:
Plasmids and phages are used as _____ for cloning purposes in prokaryotes.
